Iimveliso
Iimpawu zentliziyo - iTroponin I
I-Troponin yiprotheyini elawulayo kwimicu yezihlunu kwiiseli zezihlunu, ezilawula ubukhulu becala ukutyibilika phakathi kweefilaments ezityebileyo nezibhityileyo zemisipha ngexesha lokufinyela kwe-myocardial.Iqulethwe ngamacandelo amathathu: i-troponin T (TNT), i-troponin I (TNI) kunye ne-troponin C (TNC).Ukubonakaliswa kwee-subtypes ezintathu kwi-muscle ye-skeletal kunye ne-myocardium nayo ilawulwa ngamajini ahlukeneyo.Umxholo we-cardiac troponin kwi-serum eqhelekileyo iphantsi kakhulu kunezinye i-enzymes ze-myocardial, kodwa i-concentration in cardiomyocytes iphezulu kakhulu.Xa i-membrane yeseli ye-myocardial ilungile, i-cTnI ayikwazi ukungena kwi-membrane yeseli kumjikelezo wegazi.Xa iiseli ze-myocardial zihlanjululwa kunye ne-necrosis ngenxa ye-ischemia kunye ne-hypoxia, i-cTnI ikhutshwa egazini ngeembrane zeseli ezonakeleyo.I-concentration ye-cTnI iqala ukunyuka kwiiyure ze-3-4 emva kokuvela kwe-AMI, iphakamileyo kwiiyure ze-12-24, kwaye iyaqhubeka kwiintsuku ze-5-10.Ngoko ke, ukuzimisela kwe-cTnI yoxinaniso egazini kuye kwaba ngumqondiso ofanelekileyo wokuqwalaselwa kokusebenza kokuphindaphinda kunye nokuphindaphinda kwizigulane ze-AMI.I-cTnI ayinayo kuphela into ecacileyo eyomeleleyo, kodwa inovakalelo oluphezulu kunye nexesha elide.Ngoko ke, i-cTnI ingasetyenziselwa njengophawu olubalulekileyo ekuncedeni ukuxilongwa kokulimala kwe-myocardial, ngokukodwa i-acute myocardial infarction.
Amacandelo Angundoqo
| Microparticles (M): | 0.13mg/ml Microparticles ezidityaniswe ne-anti troponin I ultra antibody |
| I-Reagent 1 (R1): | 0.1M Tris buffer |
| I-Reagent 2 (R2): | 0.5μg/ml I-Alkaline phosphatase ebhalwe i-antitroponin I ultra |
| Isisombululo sokucoca: | 0.05% i-surfactant, 0.9% i-Sodium chloride buffer |
| Inkqutyana: | AMPPD kwi-AMP buffer |
| Isilinganisi (ukhetho): | Troponin I ultra antigen |
| Izixhobo zokulawula (ukhetho): | Troponin I ultra antigen |
Phawula:
I-1.Izixhobo azitshintshi phakathi kweebhetshi ze-reagent strips;
2.Bona ileyibhile yebhotile yecalibrator yoxinaniso lwecalibrator;
3.Jonga ileyibhile yebhotile yokulawula uluhlu loxinaniso lolawulo.
Ukugcinwa kunye nokuSebenza
1.Ugcino: 2℃～8℃, kunqanda ukukhanya kwelanga ngqo.
I-2.Ukuqinisekiswa: iimveliso ezingavulwanga zivumelekile kwiinyanga ze-12 phantsi kweemeko ezichaziweyo.
3.Iikhalibrari kunye nolawulo emva kokunyibilika kunokugcinwa iintsuku ezili-14 kwindawo eyi-2℃～8℃ emnyama.
Isixhobo Esisebenzayo
Inkqubo ye-CLIA ezenzekelayo ye-Illumaxbio (lumiflx16, lumiflx16s, lumilite8, lumilite8s).
